New organoruthenium metallates containing ferrocenecarboxalidine thiosemicarbazones and their nucleic acid/albumin binding and in vitro cytotoxicity

A string of four new hetero binuclear Ru(III) complexes of ferrocenecarboxaldehyde-4(N)-substituted thiosemicarbazones were synthesized and characterized by various spectral (infrared, ultraviolet–visible, Electron Paramagnetic Resonance (EPR) and High Resolution Mass Spectrometry (HR-MS) techniques. The binding abilities of the ligands/complexes with nucleic acid (calf thymus DNA, CT-DNA) and bovine serum albumin (BSA) were analyzed by absorption and emission titration methods. The complexes exhibited better DNA binding affinity than their parent ligands. The interaction with CT-DNA was found to be intercalative and with BSA static quenching mechanism was observed. All the synthesized Ru(III) complexes were subjected to study their in vitro cytotoxicity against MCF-7 (human breast cancer) and HT-29 (human colon cancer) cell lines. Among the four complexes, complex 3 [RuCp (FF-etsc)PPh₃]Cl exhibited the highest cytotoxicity in MCF-7 cells and complex 4 [RuCp (FF-ptsc)PPh₃]Cl was the most active on HT-29 cells.     

Micro enzymatic assay coupled to capillary electrophoresis via liquid junction

Summary A system for capillary electrophoresis combined with enzymatic assay has been evaluated for the two enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Instrumentation included a post-column reactor coupled to the separation capillary by a liquid junction. A technique for generating a substrate solution flow into the reactor by utilizing two high voltage supplies is proposed. This method offers a high degree of freedom in optimizing the separation and enzymatic reaction conditions individually. Possibilities for improving the enzymatic assay sensitivity were also examined.     

一种可实时化的多光谱图像融合系统

在多光谱摄影和光电成像技术基础上，本文提出了一种可实时化的多光谱图像融合系统。与多光谱摄影相比，不仅可实现多光谱实时化摄像，而且光谱响应进一步向近红外延伸，并引入图像处理技术。实验表明：这种技术对于增加系统的识别能力具有明显的作用，可进一步构成不同用途的新型多光谱图像融合系统。     

实时任务动态调度算法

任务调度算法是提高多任务系统效率的一种有效途径,特别是在时间紧迫环境下,实时任务调度算法更具有重要的应用价值.在研究建立实时任务调度模型方法的基础上,对模型进行理论分析,提出了实时任务调度的算法,并通过实例加以验证.     

多自由度测试技术的发展

介绍目前较常用的多种多自由度测试技术，给出每种测试技术的测试原理和应用场合，比较各种技术的优缺点和适用范围，讨论该技术发展的方向。     

槲寄生蛋白注射液细胞毒活性测定的MTT方法探讨

采用MTT比色法对体外培养的肿瘤细胞进行细胞毒作用实验，验证槲寄生蛋白注射液体外抗肿瘤效果．并且鉴定这种检测方法的有效性。实验结果表明槲寄生蛋白注射液有一定的体外抗肿瘤效果；MTT比色法是一种可用的体外细胞毒作用检测法，为新药品的开发提供了实验依据。     

可调实时时间差电子错位散斑技术及应用

本文首次提出一种可调实时时间差电子错位散斑干涉技术,并成功地用于热变形测量,该方法还可用于长时间连续变形和瞬态大变形的位移测量。叙述了该技术的原理,给出了一些应用的实验结果。     

Comparison of the sensitivity of 11 crosslinked hyaluronic acid gels to bovine testis hyaluronidase

Crosslinked hyaluronan gels are used in various applications where their stability is a prerequisite. The sensitivity of such gels to hyaluronidase can be determined as an index of stability by several approaches: chromatography, electrophoresis, and viscometry. We describe here a test based on the colorimetric determination of the N-acetyl-d-glucosamine released by hyaluronidase in standardized conditions. The sensitivities to bovine testicular hyaluronidase of 11 different gels used to fill skin wrinkles (Restylane; Perlane; Juvéderm 18, 24, 24HV, 30, and 30HV; Surgiderm 18, 24XP, 30, and 30XP) were compared.The method was reproducible, easy to perform, not time-consuming and allowed us to demonstrate that the sensitivity to testicular hyaluronidase was dependent on the degree of crosslinking of the gels and also on their monophasic/biphasic nature. Under our conditions, Surgiderm 30, 24XP and 30XP were the most resistant gels.We propose to retain the hyaluronidase test to predict the in situ stability of a crosslinked gel used to fill skin wrinkles.     

成像光谱技术中机上实时数据压缩方法研究

根据成像光谱仪的特点用一系列串行的一维压缩实现二维图像压缩.提出了在机上对原始数据从光谱方向进行实时压缩的“二真值线性预测方法”.该法可做到压缩/复原后光谱特征信息不丢失.实验表明,对大多数光谱数据在1%的重建精度下,至少可获得2:1的压缩比.从总体而言,压缩后原始数据率可降低3～4倍.文中还对压缩数据的编码和误差进行了讨论.